徐坤

基本信息

徐坤，男，1985年07月生，河南省周口市淮阳县人，遗传学博士、西北农林科技大学副教授，硕士生导师。

工作经历

2018.01-至今 西北农林科技大学动物科技学院 副教授

2015.06-2017.12 西北农林科技大学动物科技学院 讲师

科学研究

动物生物技术，主要从事动物基因编辑技术和口服酵母营养免疫调控技术的开发及应用研究；主讲本科生《分子生物学》《动物生物化学实验》和研究生《动物基因工程技术》《动物繁殖理论与生物技术》等课程；招生专业动物遗传育种与繁殖和动物营养与饲料科学。

代表性论文

[1] Ren Chonghua^#, Xu Kun^#, David Jay Sega, Zhang Zhiying*. Strategies for the enrichment and selection of genetically modified cells. Trends Biotechnol. 2019 Jan; 37(1):56-71.

[2] Li Xinyi, Bai Yichun, Cheng Xinzhen, Peter Girgis Tawfek Kalds, Sun Bing, Wu Yun, Lyu Huijiao, Xu Kun*, Zhang Zhiying*. Efficient SSA-mediated precise genome editing using CRISPR/Cas9. FEBS J. 2018 Sep; 285(18):3362-75. (Editor’s Choice)

[3] Xu Kun, Ren Chonghua, Liu Zhongtian, Zhang Tao, Zhang Tingting, Li Duo, Wang Ling, Yan Qiang, Guo Lijun, Shen Juncen, Zhang Zhiying*. Efficient genome engineering in eukaryotes using Cas9 from  Streptococcus thermophilus . Cell Mol Life Sci. 2015 Jan; 72(2):383-99. (GMD Key Scientific Article)

[4] Ren Chonghua^#, Xu Kun^#, Liu Zhongtian, Shen Juncen, Han Furong, Chen Zhilong, Zhang Zhiying*. Dual-reporter surrogate systems for efficient enrichment of genetically modified cells. Cell Mol Life Sci. 2015 Jul; 72(14):2763-72.

[5] Shao Simin, Ren Chonghua, Liu Zhongtian, Bai Yichun, Chen Zhilong, Wei Zehui, Wang Xin, Zhang Zhiying*, Xu Kun*. Enhancing CRISPR/Cas9-mediated homology directed repair in mammalian cells by expressing Saccharomyces cerevisiae Rad52. Int J Biochem Cell Biol. 2017 Nov; 92:43-52.

[6] Hafiz Muhammad Zakria, Han Baoquan, Yue Feng, Mu Lu, Fang Yuanyuan, Li Xiaoyu, Xu Kun*, Zhang Zhiying*. Significant body mass increase by oral administration of a cascade of shIL21-MSTN yeast-based DNA vaccine in mice. Biomedicine & Pharmacotherapy. 2019 Oct; 118:109147.

[7] Han Baoquan^#, Xu Kun^#,*, Liu Zhongtian, Ge Wei, Shao Simin, Li Pengcheng, Yan Nana, Li Xinyi, Zhang Zhiying*. Oral yeast-based DNA vaccine confers effective protection from Aeromonas hydrophila infection on Carassius auratus. Fish Shellfish Immunol. 2019 Jan; 84:948-54.

联系方式

通讯地址：陕西杨凌西农路22号西农大北校区动科楼225办公室

QQ：564737724

Email：xukunas@nwafu.edu.cn